|
KMID : 0043320130360070880
|
|
Archives of Pharmacal Research
2013 Volume.36 No. 7 p.880 ~ p.889
|
|
|
Inhibitory action of salicylideneamino-2-thiophenol on NF-κB signaling cascade and cyclooxygenase-2 in HNE-treated endothelial cells
|
|
Park Seong-Joon
Sung Bo-Kyung
Jang Eun-Ji
Kim Dae-Hyun
Park Chan-Hum
Choi Yeon-Ja
Ha Young-Mi
Kim Mi-Kyung
Kim Nam-Deuk
Yu Byung-Pal
Chung Hae-Young
|
|
|
Abstract
|
|
|
|
In the present study, the anti-inflammatory effect of salicylideneamino-2-thiophenol (SAL-2), a derivative of salicylate, on a potent oxidant 4-hydroxynonenal (HNE)-induced oxidative stress was investigated using rat prostate endothelial (YPEN-1) cells. We focused on anti-inflammatory activity of SAL-2 which was determined by its ability to suppress COX-2 and iNOS gene expression through suppression of NF-κB and redox regulation. We found that SAL-2 effectively inhibited HNE-induced reactive species generation, while upregulated GSH/GSSG ratio. Prostagrandin (PG) E2 production stimulated by arachidonic acid was suppressed by SAL-2. SAL-2 also downregulated COX-2 and iNOS expression induced by HNE, but salicylate did not. We found that SAL-2 inhibited HNE-mediated IKK phosphorylation, IκBα degradation and nuclear translocation of p65 which are linked to NF-κB activation. Furthermore, SAL-2 inhibited HNE-induced activation of mitogen-activated protein kinases. Collectively, SAL-2 inhibited COX-2 and iNOS gene expression through suppression of NF-κB leading to the inhibition of PGE2 synthesis. Based on these data, we propose that with its combined effect on strong anti-oxidant and anti-inflammatory action, SAL-2 can be a potent anti-inflammatory agent for treatment of inflammatory-related diseases.
|
|
|
KEYWORD
|
|
|
NF-κB, Reactive species, HNE, Inflammation, Salicylate, Salicylideneamino-2-thiophenol
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
  
|
|